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IUTA

swMATH ID: 27646
Software Authors: Niu, L., Huang, W., Umbach, D. M., Li, L.
Description: IUTA: a tool for effectively detecting differential isoform usage from RNA-Seq data. We present a new method IUTA that is designed to test each gene in the genome for differential isoform usage between two groups of samples. IUTA also estimates isoform usage for each gene in each sample as well as averaged across samples within each group. IUTA is the first method to formulate the testing problem as testing for equal means of two probability distributions under the Aitchison geometry, which is widely recognized as the most appropriate geometry for compositional data (vectors that contain the relative amount of each component comprising the whole). Evaluation using simulated data showed that IUTA was able to provide test results for many more genes than was Cuffdiff2 (version 2.2.0, released in Mar. 2014), and IUTA performed better than Cuffdiff2 for the limited number of genes that Cuffdiff2 did analyze. When applied to actual mouse RNA-Seq datasets from six tissues, IUTA identified 2,073 significant genes with clear patterns of differential isoform usage between a pair of tissues. IUTA is implemented as an R package and is available at http://www.niehs.nih.gov/research/resources/software/biostatistics/iuta/index.cfm.
Homepage: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4195885/
Dependencies: R
Related Software: BaySeq; DESeq2; rSeqDiff; EBSeq; TopHat; ShrinkBayes; edgeR; Bioconductor
Cited in: 1 Document

Cited in 1 Serial

1 Bayesian Analysis

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