FUNNEL swMATH ID: 34200 Software Authors: Zhang, Y., Topham, D. J., Thakar, J., Qiu, X. Description: FUNNEL-GSEA: FUNctioNal ELastic-net regression in time-course gene set enrichment analysis. Results: We propose an inferential framework for GSEA based on functional data analysis, which utilizes the temporal information based on functional principal component analysis, and disentangles the effects of overlapping genes by a functional extension of the elastic-net regression. Furthermore, the hypothesis testing for the gene sets is performed by an extension of Mann-Whitney U test which is based on weighted rank sums computed from correlated observations. By using both simulated datasets and a large-scale time-course gene expression data on human influenza infection, we demonstrate that our method has uniformly better receiver operating characteristic curves, and identifies more pathways relevant to immune-response to human influenza infection than the competing approaches. Availability and Implementation: The methods are implemented in R package FUNNEL, freely and publicly available at: https://github.com/yunzhang813/FUNNEL-GSEA-R-Package. Homepage: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5939227/ Source Code: https://github.com/yunzhang813/FUNNEL-GSEA-R-Package Dependencies: R Related Software: PathCellNet Cited in: 2 Publications all top 5 Cited by 6 Authors 1 Cai, Xue Hong 1 Fang, Yanning 1 Li, Xue 1 Meng, YuXiu 1 Wang, LiPei 1 Yang, Wenhong Cited in 1 Serial 2 Computational & Mathematical Methods in Medicine Cited in 2 Fields 2 Biology and other natural sciences (92-XX) 1 Statistics (62-XX) Citations by Year